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EIN3-LIKE1, MYB1, and ETHYLENE RESPONSE FACTOR3 act in a regulatory loop that synergistically modulates ethylene biosynthesis and anthocyanin accumulation

First author:Jian-Ping An;Affiliations:Shandong Agricultural University(山東農業大學):Taian,China

Corresponding author:Yu-Jin Hao

Ethylene regulates climacteric (躍變型) fruit ripening, and EIN3-LIKE1 (EIL1) plays an important role in this process. In apple (Malus domestica), fruit coloration is accompanied by (伴隨著) ethylene release during fruit ripening, but the molecular mechanism that underlies these two physiological processes is unknown. In this study, we found that ethylene treatment markedly induced fruit coloration as well as the expression ofMdMYB1, a positive regulator of anthocyanin (花青素) biosynthesis and fruit coloration. In addition, we found that MdEIL1 directly bound to the promoter ofMdMYB1and transcriptionally activated its expression, which resulted in anthocyanin biosynthesis and fruit coloration. Furthermore, MdMYB1 interacted with the promoter ofETHYLENE RESPONSE FACTOR3(MdERF3), a key regulator of ethylene biosynthesis, thereby providing a positive feedback for ethylene biosynthesis regulation. Overall, our findings provide insight into a mechanism involving the synergistic interaction (協同互作) of the ethylene signal with the MdMYB1 transcription factor to regulate ethylene biosynthesis and fruit coloration in apple.

乙烯調控呼吸躍變型的果實成熟，EIL1基因在該過程中扮演重要的作用。在蘋果中，果實成熟期的果實著色伴隨著乙烯的釋放，然而這兩個生理過程潛在的分子層面的聯繫還不清楚。本文，作者發現了乙烯處理能夠顯著誘導果實的著色以及一個花青素生物合成和果實著色正向調控的基因MdMYB1的表達。另外，作者還發現MdEIL1可以直接結合到MdMYB1基因的啟動子區，轉錄激活MdMYB1基因的表達，進而導致了花青素的生物合成和果實著色。此外，MdMYB1能夠與MdERF3基因啟動子互作，而MdERF3基因是乙烯生物合成的一個關鍵調控子，因此構成了對乙烯生物合成的正向反饋調節。綜上，本文的結果揭示了乙烯信號與MdMYB1轉錄因子的協同互作，共同調控蘋果中的乙烯生物合成和果實著色。

通訊：郝玉金(http://yyxy.sdau.edu.cn/2012/0528/c1457a15086/page.htm)

個人簡介：1994年，山東農業大學，學士；1997年，山東農業大學，碩士；2000年，華中農業大學，博士；先後在北京大學、日本果樹研究所（筑波）和美國加州大學河濱分校從事博士後研究。

研究方向：1、蘋果果實品質形成：果實色澤和酸度等品質性狀相關基因的分離和鑒定，探討溫度、光照和碳氮等信號途徑影響果實品質形成的分子機理，解析品質形成的調控網路。2、蘋果砧木抗性等研究：克隆鑒定蘋果砧木抗性等重要性狀的相關基因，揭示其作用機理，並用於砧木遺傳改良。

doi: https://doi.org/10.1104/pp.18.00068

Journal:Plant Physiology

First Published: 20 June, 2018

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